Composite

Part:BBa_K2217026:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-25)


Cas9 non-coding RNA regulation plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2683
    Illegal NheI site found at 6257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4962
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5904
    Illegal BsaI.rc site found at 5881


Design Notes

Information on the design of this part can be found at [http://2017.igem.org/Team:AFCM-Egypt/Design# Egypt-AFCM Design]


Source

Synthesized Fragments assembled by Gibson assembly

References