Composite
Part:BBa_K2217026:Design
Designed by: Mohammad Tarek Mansour Group: iGEM17_AFCM-Egypt (2017-09-25)
Cas9 non-coding RNA regulation plasmid
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2683
Illegal NheI site found at 6257 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4962
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5904
Illegal BsaI.rc site found at 5881
Design Notes
Information on the design of this part can be found at [http://2017.igem.org/Team:AFCM-Egypt/Design# Egypt-AFCM Design]
Source
Synthesized Fragments assembled by Gibson assembly